Figure 5.

RSU-1 functions in muscle to prevent illegitimate clustering of extrasynaptic L-AChRs. A, Transgenic lines were generated for the different transgenes (not drawn to scale) in the rsu-1(kr251) background. Rescue was established based on both the absence of ectopic L-AChR clusters and the presence of diffused extrasynaptic L-AChR. The two last constructs were used to determine the rsu-1 expression pattern and RSU-1 subcellular localization detailed in Figure 2, A and B. B, Confocal projections of the SABD region of an rsu-1(kr251) young adult expressing the L-AChR reporter and RSU-1-GFP fusion protein. Scale bars, 10 μm.