Figure 6.

L-AChR ectopic clusters form progressively during development in rsu-1 mutant. A, Confocal projections of the SABD region of wild-type and rsu-1(kr251) animals at L1, L2, and L3 stages expressing a presynaptic marker (VAMP, jsIs42[punc-4::snb-1::GFP]) and the L-AChR reporter (unc-29::tagRFP). Synaptic clusters are between arrowheads. Ectopic L-AChR clusters with a fluorescence level similar to synaptic clusters are detected in rsu-1(kr251) larvae. Scale bars, 5 μm. B, Proportion of wild-type and rsu-1(kr251) animals at each stage presenting zero, one, between one and 10, or >10 ectopic clusters. N indicates the number of worms. The red-hot look up table was applied on full stacks covering the entire head width, and ectopic clusters were counted when reaching a level of fluorescence similar to that of synaptic clusters.