Skip to main content
. 2016 Jun 15;36(24):6525–6537. doi: 10.1523/JNEUROSCI.3733-15.2016

Figure 7.

Figure 7.

rsu-1 ectopic L-AChR clusters are properly inserted in the plasma membrane and mainly localize in muscle arms. A, Surface detection of myc-tagged UNC-29 L-AChR subunits. Surface receptors were labeled with Cy3-conjugated anti-myc antibodies injected into unc-29::myc knock-in worms. B, Left, Schematic representation of muscle arms projected toward the nerve ring (blue) by head muscle cells (in different shades of green). Pharynx, Gray; intestine, pink. Right, Cross-section of the boxed region. SABD, Cyan. Top and bottom dashed lines represent focal planes visualized in C. C, Confocal projections of the SABD region of wild-type and rsu-1(kr251) young adults expressing a muscle membrane marker (Pmyo-3::gpi:yfp, green) and the L-AChR reporter (red). Shown are projections of 0.4 μm depth with a gap of 2.4 μm between top and bottom planes for each genotype. Arrows point to rsu-1(kr251) ectopic L-AChR clusters colocalized with muscle arm membrane. Arrowheads delimit the synaptic region. Scale bars, 10 μm.