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. 2016 Jun 15;36(24):6525–6537. doi: 10.1523/JNEUROSCI.3733-15.2016

Figure 8.

Figure 8.

Ectopic L-AChR clusters result from the auto-assembly of the L-AChR associated scaffold. A, Confocal projections of the SABD region of fixed wild-type and rsu-1(kr251) young adults. Immunofluorescence staining of LEV-9::T7 using anti-T7 antibodies and of L-AChRs using anti-UNC-38 antibodies. The LEV-9 scaffolding protein colocalizes with rsu-1(kr251) ectopic L-AChR clusters. B, L-AChR distribution (unc-29::tagRFP knock-in) in the same region of single and double mutants. rsu-1(kr251) synaptic and ectopic L-AChR clusters are not observed in the double-mutant contexts. C, Confocal projections of the SABD region of control and rsu-1(kr251) young adults expressing the L-AChR reporter in a madd-4(kr249) background rescued by expression of Ce-Punctin long isoforms fused to GFP. Ce-Punctin-L localization is not impaired and is restricted to synapses in the rsu-1 mutant. Arrowheads delimit the synaptic region. Scale bars, 10 μm.