Figure 3.

GPCs produce OPC-type neurons when Wnt signaling is suppressed. A, The lineages of GPCs were labeled by LacZ under the control of wg-Gal4 UAS-CD8GFP (green), UAS-flp, and act>stop>lacZ. LacZ expression (blue) was detected in a subset of Eya⁺ cells (magenta, arrows). B, When the dominant negative form of Dfz2 was expressed under the control of wg-Gal4, lacZ expression was not detected in the innermost region of the medulla cortex (white arrows). LacZ (blue) and Drf (magenta) double-positive neurons were observed within GPCs (black arrowheads). B′, Magnified images of the areas indicated by yellow squares in B.