Figure 1.

Olig2 is an important transcription factor in LPC-injured oligodendrocytes. A, Schematic overview of oligodendrocyte (OL) sample preparation for Chip-Seq analysis. Mature oligodendrocytes were cultured and treated with LPC for 12 h before chromatin immunoprecipitation. B, Heat maps of the H3K27Ac signals in the vehicle-treated and LPC-treated oligodendrocytes. Each line on the y-axis represents a genomic region of ±2.5 kb flanking the H3K27Ac peaks. C, Genomic distribution of the H3K27Ac peaks in vehicle-treated and LPC-treated oligodendrocytes. TSS, Transcription start site; TTS, transcription termination site. D, GO analysis of the differentially H3K27Ac-bound genes. E, The transcription factors with the most significant differential in intensity of H3K27Ac-bound peaks as determined by the tag numbers with and without LPC treatment. F, Genome browser view of the distribution of H3K27Ac on Olig2, Gmeb2, Psip1, and Gapdh. G, Quantitative real-time PCR analysis of the Olig2 mRNA levels in vehicle oligodendrocytes and oligodendrocytes treated with 12.5 mm LPC for 12 h. Data represent the means ± SEM from three independent experiments. **p < 0.01, Student's t test. Veh, Vehicle.