. 2016 Sep 14;36(37):9535–9546. doi: 10.1523/JNEUROSCI.3730-15.2016

Table 3.

Shock reactivity and olfactory acuity of flies expressing RNAi-Nep(A) under the control of the MB-Gal80;c316 driver

Genotype	Shock reactivity	Olfactory acuity
Genotype	Shock reactivity	Octanol	Methylcyclohexanol
MB-Gal80;c316/+	60.80 ± 3.405	60.63 ± 6.219	60.90 ± 6.667
MB-Gal80;c316/Nep1A	58.30 ± 1.808	67.10 ± 7.114	58.70 ± 5.173
+/Nep1A	64.10 ± 4.927	62.10 ± 5.288	59.50 ± 5.300
MB-Gal80;c316/+	63.25 ± 4.366	52.36 ± 7.890	65.25 ± 7.345
MB-Gal80;c316/Nep2A	62.83 ± 3.946	47.17 ± 5.455	73.13 ± 4.155
+/Nep2A	77.67 ± 3.530	60.15 ± 4.650	69.13 ± 8.532
MB-Gal80;c316/+	58.43 ± 5.149	49.00 ± 4.472	54.56 ± 3.986
MB-Gal80;c316/Nep3A	52.50 ± 4.056	54.75 ± 6.648	53.42 ± 4.763
+/Nep3A	73.70 ± 4.261	51.17 ± 6.457	54.33 ± 3.945
MB-Gal80;c316/+	58.57 ± 4.961	52.33 ± 4.330	58.56 ± 4.628
MB-Gal80;c316/Nep4A	56.90 ± 8.849	52.00 ± 4.424	62.00 ± 4.149
+/Nep4A	58.30 ± 5.863	53.08 ± 5.016	52.08 ± 3.718

Data are shown as means ± SEM. All genotypes tested exhibit normal shock reactivity (Nep1A: F_(2,29) = 0.6487, p = 0.5307, n = 10; Nep2A: F_(2,35) = 4.544, p = 0.0181, n = 12, post-hoc Newman–Keuls test, MB-Gal80;c316/Nep2A vs +/Nep2A *p < 0.05; Nep3A: F_(2,26) = 6.699, p = 0.0049, n ≥ 7, post hoc Newman–Keuls test, MB-Gal80:c316/Nep3A vs +/Nep3A **p < 0.01; Nep4A: F_(2,26) = 0.01578, p = 0.9844, n ≥ 7) and normal olfactory acuity for octanol (Nep1A: F_(2,27) = 0.2913, p = 0.7498, n ≥ 8; Nep2A: F_(2,35) = 1.249, p = 0.3000, n ≥ 11; Nep3A: F_(2,32) = 0.2108, p = 0.8111, n ≥ 9; Nep4A: F_(2,32) = 0.01515, p = 0.9850, n ≥ 9) and methylcyclohexanol (Nep1A: F_(2,29) = 0.03746, p = 0.9633, n = 10; Nep2A: F_(2,23) = 0.3230, p = 0.7275, n = 8; Nep3A: F_(2,32) = 0.01955, p = 0.9807, n ≥ 9; Nep4A: F_(2,32) = 1.609, p = 0.2168, n ≥ 9).