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. 2016 Dec 7;36(49):12425–12435. doi: 10.1523/JNEUROSCI.2016-16.2016

Figure 3.

Figure 3.

PHF1 is expressed primarily in hippocampal neurons of P301S male mice treated with AAVrh.10PHF1 vector. A–H, PHF1 is primarily colocalized to NeuN-positive neurons (A–D) but not GFAP-positive astrocytes (D–H). A, A representative photomicrograph of double-staining IHC using an anti-mouse antibody (green) and NeuN antibody (red) and hippocampal sections from P301S mice treated with AAVrh.10PHF1. B, C, Higher-power images of PHF1 (B) and NeuN (C) expression from the boxed area of A. D, A merged image of B and C. E, A representative photomicrograph of double-staining IHC using an anti-mouse antibody (green) and GFAP antibody (red) and of hippocampal sections of P301S mice treated with AAVrh.10PHF1. F, G, Higher-power images of PHF1 (F) and GFAP (G) expression in the boxed area of E. H, A merged image of F and G. I, Quantitative analysis of the subiculum of AAVrh.10PHF1-treated P301S mice (n = 12) demonstrated that 95.5 ± 0.009% of PHF1-expressing cells are NeuN-positive neurons, whereas only 2.12 ± 0.32% of PHF1-expressing cells are GFAP-positive astrocytes.