Figure 4.

5-HT prevents AβO-induced nuclear translocation of NFκB (p65 subunit) in microglia. A–L, Microglial cultures were pretreated with 1 μm 5-HT for 30 min (as indicated) before the addition of 100 nm AβOs (or vehicle) and incubation for 2 h. Cells were fixed, permeabilized, and incubated with anti-NFκB p65 (red) and DAPI (blue). Scale bar, 50 μm. M, NFκB p65-positive nuclei were counted and are shown relative to total nuclei. Error bars indicate means ± SEM; n = 5 independent microglial cultures, 2–3 coverslips per experimental condition per culture, 10 images acquired per coverslip; two-way ANOVA followed by Dunnett's multiple-comparisons test, *p ≤ 0.05.