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. 2016 Feb 3;36(5):1456–1470. doi: 10.1523/JNEUROSCI.2390-15.2016

Figure 5.

Figure 5.

5-HT3A and P2X2 receptors colocalize in common surface clusters. A, 5-HT3AR-HA was transfected alone or cotransfected with P2X2R-YFP in hippocampal neurons at 7 DIV. Immunofluorescence detection of both receptors (red represents 5-HT3AR-HA; green represents P2X2-YFP) was performed after 48 h. The presence of transfected P2X2R in the same neurons enhanced the distal trafficking of 5-HT3AR in all neurites, particularly in axons (arrows). Scale bar, 50 μm. B, Magnification of an axonal section reveals that 5-HT3AR and P2X2R colocalize in common surface clusters (arrowheads). C, Fluorescence profiles of both labels along a polyline drawn through the surface clusters (boxed section). Scale bar, 5 μm.