Figure 8.

Dendritic length and complexity are increased by chronic CORT and FLX treatments. A, Experimental timeline of viral injections and analysis. B, Confocal images of vehicle, CORT, and CORT+FLX-treated OB slices showing GFP⁺ neurons and DAPI staining. Scale bar, 20 μm. C, Lentiviral vectors encoding GFP under the control of the CMV promotor were injected in the RMS. D, FLX treatment alone increased the number of dendrites in newborn OB neurons. E, CORT+FLX and FLX treatments significantly increased dendritic length. F, Graph depicting the effect of chronic CORT, CORT+FLX and FLX treatments on the number of dendritic branches, according to Sholl analysis. CORT+FLX treatment increased the number of branches. Values are plotted as mean ± SEM (n = 4/group). *p < 0.05, **p < 0.01, ***p < 0.001 in CORT versus vehicle; #p < 0.05, ##p < 0.01 in CORT versus CORT+FLX; §p < 0.05 in vehicle versus FLX.