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. 2019 Jun 27;13:2097–2110. doi: 10.2147/DDDT.S195954

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© 2019 Tsagogiorgas et al.

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Phagocytosis. Cellular uptake of DiI-F6H8-Pluronic or DiI-F6H8-lecithin emulsions. In these emulsions, F6H8 was fully saturated with the lipophilic DiI and then further processed for emulsification. Peripheral blood mononuclear cell (PBMC) (10⁶ cells/mL) were incubated for 24 hrs with DiI-F6H8-lecithin (panel to the left) or DiI-F6H8-Pluronic (panel to the right) emulsions (4%, w/v). Thereafter, cytospins were prepared and the cells were analyzed by fluorescence microscopy. Original magnification: 400x. White bar: 20 µm. Blue: nucleus of PBMCs; red: phagocytized DiI-F6H8 emulsion droplets.