KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological Samples
Fava bean plants (Viciafaba), Improved Long Pod variety	Harris Seeds, Rochester NY	Cat#00096-00-01-330
Chemicals, Peptides, and Recombinant Proteins
RNAlater-ICE Frozen Tissue Transition Solution	ThermoFisher Scientific	Cat#4427575
Trizol Reagent	ThermoFisher Scientific	Cat#15596026
2-Propanol	Fisher Chemical	Cat#A416-500
EvaGreen	VWR	Cat#89138-984
Taq DNA polymerase (Invitrogen)	Invitrogen via Fisher Scientific	Cat#10-342-053
Critical Commercial Assays
RNA Clean & Concentrator-5 (DNAse included)	Zymo Research	Cat#R1013
TruSeq Stranded mRNA Library Prep Kit	Illumina	Cat#RS-122-2101
iScript™ cDNA Synthesis Kit	BioRad USA	Cat#1708890
Phusion® High-Fidelity PCR Master Mix with HF Buffer	New England Biolabs	Cat#M0531S
MEGAscript™ RNAi Kit	ThermoFisher Scientific	Cat#AM1626
NEBNext® Poly(A) mRNA Magnetic Isolation Module	New England Biolabs	Cat#E7490S
SuperScript™ IV First-Strand Synthesis System	Invitrogen via ThermoFisher Scientific	Cat#18091200
Oligonucleotides
qPCR Apns-1 F:	GCAAACGTCGTTTCTGCCTT
qPCR Apns-1 R:	ACGACTACGAATCTGGCACG
qPCR Apns-2 F:	AGTATCCTTGTTGTCCGCCC
qPCR Apns-2 R:	GCGCACCAATTCCTAAGAGC
qPCR G3PDH F:	CGGGAATTTCATTGAACGAC
qPCR G3PDH F:	TCCACAACACGGTTGGAGTA
qPCR NADH F:	CGAGGAGAACATGCTCTTAGAC
qPCR NADH R:	GATAGCTTGGGCTGGACATATAG
RNAi Apns F:	TAATACGACTCACTATAGGGTCCGTTTCAATAGCTTCCGAA
RNAi Apns R:	TAATACGACTCACTATAGGGACTGCTGCACCGATGAAGAA
Software and Algorithms
R	https://www.r-project.org/	v.3.5.0
Cutadapt	https://cutadapt.readthedocs.io/en/stable/	v.1.2.1
fastq-mcf (ea-utils)	https://expressionanalysis.github.io/ea-utils/	v.1
tophat	https://ccb.jhu.edu/software/tophat/index.shtml	v.2
htseq	https://htseq.readthedocs.io/en/release_0.11.1/	v.1
edgeR	https://bioconductor.org/packages/release/bioc/html/edgeR.html	v.3.18.1
TranslatorX	http://www.translatorx.co.uk/?COLLCC=3253199103&
IQ-TREE	http://iqtree.cibiv.univie.ac.at/