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. Author manuscript; available in PMC: 2020 Jan 3.
Published in final edited form as: Cell Stem Cell. 2018 Nov 21;24(1):153–165.e7. doi: 10.1016/j.stem.2018.10.016

Figure 1. IKZF2 is required for leukemogenesis but dispensable for normal hematopoiesis.

Figure 1.

(A) Experimental scheme for investigating normal and transformed LSK cells. Leukemia initiation experiment was performed using the murine MLL-AF9 model. (B) Ikzf2 is dispensable for colony-forming ability of normal stem cells. Colony Assay was performed with LSK cells from Ikzf2f/f n=4 and Ikzf2Δ/Δ n=4 mice (C) Ikzf2 deletion in MLL-AF9 transformed LSK cells reduced colony formation. MLL-AF9 transformed LSK cells from Ikzf2f/f n=3 and Ikzf2Δ/Δ n=3 mice were used for colony assay. Mean +/− S.E.M of three independent experiments, Student’s t test **, p<0.01. (D) Deletion of Ikzf2 delays leukemia progression in MLL-AF9 model. Survival analysis is from the result of three combined transplants with genotypes Ikzf2f/f n=13 and Ikzf2Δ/Δ n=28 mice. **, p<0.01 logrank test. (E,F) Ikzf2Δ/Δ deleted leukemic mice have reduced disease burden. (E) Spleen and (F) liver weights of moribund Ikzf2f/f and Ikzf2Δ/Δ mice with proper deletion. Result is from Ikzf2f/f n=12 and Ikzf2Δ/Δ n=8 mice. **P < 0.01, unpaired Student’s t test. (G) QPCR of Ikzf2 in bone marrow leukemic cells from Ikzf2f/f n=12 and Ikzf2Δ/Δ n=8 mice. ****p<0.0001. unpaired Student’s t test. (H) Bone marrow leukemic cells from Ikzf2Δ/Δ mice have reduced IKZF2 expression. Frequency of IKZF2High cells in primary leukemic mice was examined by intracellular flow cytometry for IKZF2. Result from Ikzf2f/f n=12 and Ikzf2Δ/Δ n=10 mice is shown. ***, p<0.001. unpaired Student’s t test.