(A) QPCR analysis of Ikzf2 mRNA in sorted MLL-AF9 Ikzf2f/f cre-ER c-Kit High cells treated with 4-OHT for different time points. (B-E) Acute deletion of Ikzf2 in LSCs increases genes data showing that acute deletion of Ikzf2 reduces Hoxa9 and c-Myc mRNA levels. (A-H) Results shown are from four different experiments. Student’s t test *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001. (I) Western blot analysis showing reduction of IKZF2, HOXA9 and C-MYC after Ikzf2 is acutely deleted in MLL-AF9 Ikzf2f/f cre-ER High cells. (J-N) Box and whiskers plot showing correlation of the indicated genes in patients with high (n=24) versus low IKZF2 (n=45) expression in AML patients from TCGA dataset. (J) CD34 (K) C3 (L) CEBPE (M) CEBPD (N) MYC .*P < 0.05, **P < 0.01, ***P < 0.001, two-tailed t test.