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. 2019 Jun 27;28(13):860–870. doi: 10.1089/scd.2018.0247

FIG. 2.

FIG. 2.

Identification of ACSs from P3 mouse AC. (A) Primary culture of AC cells. Dissociated primary AC cells form cell clusters and grow into spheres in ∼5 days. (B) Secondary culture of AC cells. Primary cultured AC cells are dissociated and passaged into secondary AC cells, which form cell clusters and grow into spheres in ∼5 days. (C) Tertiary culture of AC cells. Secondary cultured AC cells are dissociated and passaged into tertiary AC cells, which form cell clusters and grow into spheres in ∼5 days. Scale bar: 50 μm in (A–C). ACSs, AC-derived spheres.