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. 2019 Jun 27;28(13):860–870. doi: 10.1089/scd.2018.0247

FIG. 3.

FIG. 3.

ACSs express NSC genes and proteins. (A) Confocal microscope images show that tertiary ACSs incorporate EdU (arrows). (B) Real-time PCR shows gene expression in the ACT and ACS. NSC genes Sox2 and Nes are expressed at higher levels in the ACS compared to the ACT (** indicates P < 0.01, Student's t-test). (C) Confocal microscope images show that ACSs express NSC proteins SOX2 and NESTIN in tertiary culture spheres. (D) Quantification study of SOX2 and NESTIN immunostaining in tertiary culture spheres. The percentages of NESTIN-positive, SOX2-positive, and both NESTIN- and SOX2-positive cells are 84.68% ± 6.05%, 88.59% ± 2.33%, and 81.17% ± 5.16%, respectively, in tertiary spheres (mean ± standard error; ** indicates P < 0.01, Student's t-test, n = 8 cell cultures). (E) Confocal microscope images show that ACSs express oligodendrocyte precursor marker protein A2B5 in tertiary culture sphere. Scale bar: 50 μm in (A, C, and E). ACSs, AC-derived sphere; ACT, auditory cortex tissue; EdU, 5-ethynyl-2-deoxyuridine; PCR, polymerase chain reaction.