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. 2019 Jun 27;28(13):860–870. doi: 10.1089/scd.2018.0247

FIG. 7.

FIG. 7.

ACM stimulates the synaptogenesis of ACNs by SV2 immunostaining. (A) Confocal microscope images show that TUJ1-positive connections and SV2 puncta (arrows) are found between ACNs in the ACM and control (CTRL) groups. (B) High magnification images (rectangular area in A) show SV2 puncta (arrows) along the connections in the ACM and control groups. (C) In the quantification study, the number of SV2 puncta in the ACM group is significantly higher than the control group (mean ± standard error shown in the figure; ** indicates P < 0.01, Student's t-test; n = 10 cell cultures per group). (D) The quantification study showed that the area of SV2 puncta in the ACM group is significantly larger than the control group (mean ± standard error shown in the figure; ** indicates P < 0.01, Student's t-test; n = 10 cell cultures per group). Scale bar: 20 μm in (A) and 2 μm in (B).