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. 2019 Jun 27;28(13):860–870. doi: 10.1089/scd.2018.0247

FIG. 8.

FIG. 8.

ACM stimulates synaptogenesis of ACNs by pre- and postsynaptic protein immunostaining. (A) Confocal microscope images show that TUJ1-positive connections and SYNAPSIN/PSD93 colocalized puncta (arrows) are found between ACNs in the ACM and control (CTRL) groups. (B) High magnification images (rectangular area in A) show SYNAPSIN and PSD93 puncta (arrows) along the connections in the ACM and control groups. (C) Quantification study indicates that the number of SYNAPSIN/PSD93 colocalized puncta is significantly increased in the ACM group (mean ± standard error shown in the figure; ** indicates P < 0.01, Student's t-test; n = 10 cell cultures per group). (D) Quantification study suggests that the area of SYNAPSIN/PSD93 colocalized puncta is significantly larger in the ACM group (mean ± standard error shown in the figure; **P < 0.01, Student's t-test; n = 10 cell cultures per group). Scale bar: 20 μm in (A) and 2 μm in (B).