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. 2019 Jun 27;28(13):846–859. doi: 10.1089/scd.2019.0045

FIG. 4.

FIG. 4.

Differentiation of LS-iPSCs cultures generates cells representative of ectoderm, mesoderm, and endoderm embryonic germ layer lineages. (A) LS1-iPSCs cultures do not express markers for differentiated cell types. Upon differentiation, LS1-iPSC derived cultures contain cells that are positive for markers of ectodermal (MAP2, βIIITub), mesodermal (αSMA, DES), and endodermal (VIM) lineages, indicating pluripotent differentiation potential. Scale bar = 50 μm. Image inserts show DAPI nuclear counterstain overlay. (B) Analysis of differentiated cultures by the Scorecard qPCR array demonstrates downregulation of stem cell self-renewal genes, with upregulation of genes involved in germ layer specification and differentiated cell identity. Comparison of gene expression signatures with standard control databases allows for the analysis of lineage bias of each lines differentiation potential. Differentiated LS1-iPSCs result in gene expression signatures positive for all three germ layers, exhibiting full differentiation potential. αSMA, alpha smooth muscle actin; DES, desmin; VIM, vimentin.