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Detection of mutation through PCR amplification and Sanger sequencing of MtDNA: LS (8993 T>G). Following extraction of mtDNA, detection of mutation through PCR amplification and Sanger sequencing of MtDNA: LS (8993 T>G) was conducted in (A) Parental LS1 (BJ fibroblast-control); (B) LS1 iPSC across multiple passages (BJ-iPSC-control); (C) differentiated LS1-iPSC (differentiated BJ-iPSC-control). mtDNA, mitochondrial DNA.
