Fig. 1.

Mice on a WD supplemented with SW develop obesity, fatty livers, inflammation, liver fibrosis, and steatosis. B6/129 female mice were fed a CD or WD with SW for 16 weeks. Mice were 12 weeks old at the beginning of feeding the WD + SW (n = 10), whereas the control mice group fed the CD consisted of 4 mice that were 10 weeks old and 6 that were 12 weeks old. A: Body weight. B: Liver weight. C: Hepatic triglycerides levels. D: Glucose tolerance tests. E: Histology scores for liver steatosis, inflammation, hepatocyte ballooning, NAFLD activity score, and fibrosis. F: Representative images of liver sections stained with H&E or Picrosirius red. Size bars: 10 μm. Data are expressed as means ± SEMs, n = 8–10 mice per group. *P < 0.01 and **P < 0.001.