Fig. 2.

Increased body weight and liver inflammation in mice fed an HFD for 20 weeks. A: Weight development in male HFD and STD C57BL/6 mice. B: Epididymal fat weight as a percentage of total body weight after 20-week HFD feeding in C57BL/6 mice. C: Macroscopic aspect of livers from STD and HFD C57BL/6 mice. D: Representative H/E (left), Sirius Red (middle), and Oil Red O staining (right) of STD (upper panel) and HFD (bottom panel) wild-type mice, illustrating steatosis (white arrow), necroinflammation (inserts with magnification), cellular hypertrophy (black arrow), fat accumulation (middle panel), and collagen deposition (right panel). Scale bar: 100 µm. E: Serum liver function tests (AST and ALT) in HFD and STD mice. F: Liver tissue was analyzed for mRNA expression of Tnf, normalized to Hprt. Statistical analysis: Mann-Whitney U test, n ≥ 9. ***P < 0.001, **P < 0.01, and *P < 0.05.