Fig. 1. Enzymatic activity of α-carbonic anhydrase from Persephonella marina EX-H1 (pmCA) and its inhibition by acetazolamide (AZM).

Enzymatic CO₂ hydration (A) and CaCO₃ precipitation (B), as measured by changes in pH and absorbance at 600 nm (OD₆₀₀) over time, respectively. For AZM inhibition, protein samples were mixed with varying concentrations of AZM, and changes in pH (C) and OD₆₀₀ (D) were measured as a function of time, respectively. Reactions without pmCA were prepared as negative controls. (E) Inhibition of pmCA with varying concentrations of anions. The CO₂ hydration activity without any inhibitors was used as a positive control, and was set to 100% for comparison. The enzyme activity in the presence of AZM was used as a negative control. Data points indicate the means (± standard deviation [SD]) of triplicate measurements from the same preparation. Error bars indicate SD.