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. 2019 Jul 1;14(7):e0207169. doi: 10.1371/journal.pone.0207169

Fig 5. Umbelliprenin inhibits the nuclear translocation of β-catenin in gastric cancer cells.

Fig 5

A. The translocation of β-catenin was examined by immunostaining. Cells were treated with UM (0, 6, 12, 24 μM for AGS cells or 0, 12.5, 25, 50 μM for BGC-823 cells) for 24 h, stained with the anti-β-catenin antibody and analysed using the Image Xpress Micro imaging system (MD, USA). B. Protein expressions were detected by western blot. After treatment with Umbelliprenin (0, 6, 12, 24 μM for AGS cells or 0, 12.5, 25, 50 μM for BGC-823 cells) for 24 h. Cellular fractionation was carried out to determine the cellular localization of β-catenin. Lamin B and β-actin were used as controls for nuclear fraction and cytoplasmic fraction, respectively. C. The relative expression of cytoplasmic and nuclear proteins was analyzed. The results obtained from a representative experiment are shown (n = 3). Statistical significance was **p < 0.01.