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. 2019 Jul 1;14(7):e0219143. doi: 10.1371/journal.pone.0219143

Fig 4. Murine mutant Cbl-c Δex7 prevents ubiquitination and downregulation of EGFR by WT Cbl proteins.

Fig 4

(A) Cbl-c Δex7 prevents ubiquitination of EGFR by WT Cbl. HEK 293T cells were transfected with empty vector (Control), WT Cbl, Cbl-c Δex7, or a combination of WT Cbl and Cbl-c Δex7 as indicated. All cells were cotransfected with EGFR. Cells were stimulated with EGF for either 0, 10 or 30 minutes, harvested, and immunoprecipitated with anti-EGFR antibodies. The EGFR IP and WCL were probed for proteins as indicated. Molecular weight standards in kDa are indicated on the left of each panel. (B) EGFR-associated ubiquitin was quantified and normalized to total EGFR from the EGFR IP. (C) EGFR levels were quantified from the WCL and normalized to Hsc70. (D) EGFR-associated Cbl was quantified and normalized to total EGFR from the EGFR IP (Panel 2). Graphs represent the mean from 5 experiments +/- SE. p-values for relevant comparisons are shown in the tables accompanying the graphs. Significant comparisons (p≤0.05) are highlighted in yellow.