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. 2019 Jun 5;12(6):dmm039602. doi: 10.1242/dmm.039602

Fig. 3.

Fig. 3.

Proprotein expression and activity are decreased in STT3A-null cells. (A) INSR western blot analysis of HEK WT, STT3A-null (ΔA) and STT3B-null (ΔB) cells after treatment with PCSK inhibitor Dec-RVKR. (B) PCSK activity in HEK WT cells, with and without addition of Dec-RVKR. (C) Western blot analysis of INSR in Dec-RVKR-treated cells, followed by Endo-Hf- or PNGase-F treatment of the cell lysates. (D) Quantitative PCR analysis of PCSKs in HEK cells. (E) Quantitative PCR analysis of the transcript abundance of PCSK5a and furin in the different cell lines studied (average of technical duplicates from three biological replicates). One-way ANOVA analysis was used to determine statistical significance. (F) Average total convertase activity of three biological replicates towards a furin substrate in WT, mutant and NGI-1-treated HEK cells and in LoVo cells. One-way ANOVA analysis was done to determine statistical significance. Error bars represent s.e.m. *P<0.05, **P<0.01, ***P<0.001.