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. 2019 Jun 5;12(6):dmm039602. doi: 10.1242/dmm.039602

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Ectopically expressed PCSK5a is underglycosylated in STT3A-null cells and fails to rescue the receptor-processing defects. (A) Representative western blots in HEK and LoVo cells transfected with Flag-tagged murine constructs for PCSK5a and furin. (B) Quantification of the level of pro-receptor relative to the total amount of receptor (pro-receptor+mature receptor). Average ratios from three (IGF-1R) or four (INSR) separate experiments are shown; error bars represent s.e.m. One-way ANOVA analysis was used to determine statistical significance. **P<0.01, ***P<0.001. (C) INSR western blot of PCSK5a- or furin-transfected HEK and LoVo cells, followed by Endo-H_f- or PNGase-F treatment of the cell lysates. (D) IGF-1R immunostaining of PCSK5a- and furin-transfected and untransfected STT3A-null and LoVo cells.