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Site-directed mutagenesis in PCSKa N-glycan sites does not affect rescue in LoVo cells. Site-directed mutagenesis was done on four N-glycan sites of PCSK5a and two furin N-glycan sites. Representative Flag, INSR and IGF-1R western blots of LoVo cells transfected with WT or N-glycan-site-mutated constructs for PCSK5a and furin. β-actin was used as a loading control. Blots are representative of three individual transfections.
