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. 2019 Jun 17;12(6):dmm037176. doi: 10.1242/dmm.037176

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Molecular mass of collagen VI is reduced upon loss of Colgalt1 function in MEFs. Immunoblot detection of the α1 chain of type VI collagen in lysates (A) and conditioned media (B) from wild-type and fosse MEF cultures. The double arrows indicate the difference in electrophoretic mobilities between the collagen VI molecule derived from wild-type and fosse MEF. Immunoblots were subjected to densitometric analyses with ImageJ using Ponceau S staining for normalization. (C) Immunofluorescent detection of type VI collagen in muscle tissue from wild-type and fosse mice. Magnification: 40×. (D) Phalloidin staining of wild-type and double-homozygous colgalt1 mutant zebrafish at day 9 post-fertilization reveals muscle disorganization.