Fig. 3.

Enhancement of AMP expression in the mxc^mbn1 mutant. (A,B,E,F) Bright-field images of control (w/Y) (A,E) and mxc^mbn1 (B,F) whole larvae. (C,D,G,H,I,J,K,L) Bright-field images of the larval fat bodies around the salivary glands from the control (C,G,I,K) and mxc^mbn1 (D,H,J,L) larvae, raised on normal diet (A-H) or on a diet containing antibiotics (I-L). (A′,B′) Fluorescence images of whole larvae carrying Drs-YFP in control (A′) and mxc^mbn1 (B′). (C′,D′) Fluorescence images of larval fat bodies carrying Drs-YFP from control (C′) and mxc^mbn1 larvae (D′). Notably, Drs-YFP was induced in fat bodies of the mxc^mbn1 mutant, but not in the control. (E′,F′) Fluorescence images of the whole larvae carrying Dpt-YFP in control (E′) and mxc^mbn1(F′). (G′,H′) Fluorescence images of larval fat bodies caring Dpt-YFP from control (G′) and mxc^mbn1 larvae (H′). Notably, Dpt-YFP was also induced in fat bodies of the mxc^mbn1 mutant, but not in control. (I′-L′) Fluorescence images of larval fat bodies carrying Drs-YFP (I′,J′) or Dpt-YFP (K′,L′) from control (I′,K′) and mxc^mbn1 larvae (J′,L′). Notably, Drs-YFP and Dpt-YFP were also induced in fat bodies of the mxc^mbn1 larvae raised under aseptic conditions. Scale bars: 500 μm.