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Overview of the custom-made light box chamber to detect the circadian rhythm in fish. (A) Schematic of the fish circadian rhythm chamber, which consisted of an IR camera (IR CCD), an LED light box and a temperature control incubator. (B,C) Example snapshots of fish position in the water tank during the (B) light (day) and (C) dark (night) cycles. (D) LED light source arrangement for the fish circadian rhythm chamber.
