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. 2019 Jun 10;8(6):bio041871. doi: 10.1242/bio.041871

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Comparison of circadian rhythm locomotion activities of zebrafish and catfish measured by the idTracker or ImageJ methods. (A) Schematic of the analysis pipeline for fish locomotion measurement in this study. (B,C) The circadian rhythm of diurnal fish species (B, zebrafish) and nocturnal fish species (C, catfish) identified by either idTracker (blue) or ImageJ (red). The data are expressed as the means. (D,E) Comparison of normalized percentages between idTracker and ImageJ results after data normalization in total zebrafish circadian activity in (D) day cycle and (E) night cycle with r-value (highlighted in blue) from Spearman’s nonparametric correlation test. (F,G) Comparison of normalized percentages between idTracker and ImageJ results after data normalization in total catfish circadian activity in (F) day cycle and (G) night cycle with r-value (highlighted in blue) from Spearman’s nonparametric correlation test. The data are expressed as the means±s.e.m. (zebrafish, n=18; catfish, n=6) and were analyzed by unpaired t-test with Welch's correction.