Reconstruction of developmental trajectories of the diencephalon. (A) URD-inferred hierarchical tree of diencephalic lineages. (B) Force-directed layout of the trajectory tree colored by segments as shown in A. (C) Expression of lineage markers overlaid on the trajectory tree. The arrows indicate robust expression in each specific lineage. cTh, caudal thalamus; ETh, epithalamus; PTh, prethalamus; rPT, rostral pretectum; rTh, rostral thalamus; ZLI, zona limitans intrathalamica.