Fig. 8.

Gene expression cascades involved in the development of different diencephalic lineages. (A-F) Heatmaps showing gene expression dynamics in different diencephalic lineages. Pseudotime increases from left to right. Lineage-specific genes are shown to the right. To the left are the genes that are significantly altered in the thalamus by Gbx2 deletion (red, upregulated; blue, downregulated; gray ,unchanged). (G) Temporal dynamics of 14 transcription factors that exhibit the most significant changes in the specification of rostral thalamic neurons. Each dot represents the moving-window average of expression levels; the line shows impulse response fitting: blue for genes that change at a constant rate, green for a single sigmoid impulse, and red for a convex impulse; vertical lines indicate the onset (orange) and offset (magenta) of gene expression. (H) Expression of key developmental regulators overlaid on the branch point forming the rostral thalamus (right) and the prethalamus (left), respectively. The dashed arrows indicate the lineage bifurcation and trajectory; the arrowhead shows the presence of Dlx2 expression in rostral thalamic cells near the branch point.