Fig. 5.

Left-right atrial cardiomyocyte identity defects in Pitx2 null cardiac tissue. (A) Pseudotime score for each individual outflow tract-associated transcriptome projected onto the UMAP plot. Cells not included in analysis are colored dark gray. (B) Pseudotime minimal spanning tree (MST) plots displaying pseudotime score (top) and cell identity (bottom). Cell clusters are colored according to Fig. 4A. (C) STREAM plot for indicated cell clusters. (D) Heatmap showing clusters of dynamic gene expression trends across pseudotime (left). Results of hierarchical clustering of gene expression trends shown in heatmap (middle). Gene ontology (GO) analysis of genes from each indicated dynamic cluster (right). (E) Individual gene expression trends plotted across pseudotime. Cells are colored according to their identity (Fig. 4A). muCP, progenitors enriched in Pitx2 null tissue; pSHF, posterior second heart field progenitors; RA, right atrial cells.