Fig. 6.

Pitx2-dependent valvulogenesis. (A) UMAP of cardiac valve-associated cell clusters from E10.5 and E13.5. (B) Force-directed graph of PAGA-initialized embedding derived from cells in Fig. 6A (left). PAGA graph showing the relationships between the subclusters (right). (C) Individual gene expression feature plots. Outlines indicate clusters colored according to A. (D) Dotplot showing the expression of individual marker genes across the distinct cardiac valve-associated clusters. Low average expression is colored as blue, and high average expression is denoted with red. The size of the dot represent the percent of cells within that cluster expressing the indicated gene. AVcu, atrioventricular cushion cells; CFs, cardiac fibroblasts; EndoC, endocardial cells; EndoV, endocardial valve cells; Mes, mesenchymal cells; VaMes, valve mesenchyme.