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. 2019 Mar 27;146(12):dev173807. doi: 10.1242/dev.173807

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — High throughput scRNA-seq from the developing spinal cord. (A) Cervical (orange) and thoracic (blue) regions of the spinal cord of mouse embryos from stage e9.5 to e13.5 were dissected, dissociated and sequenced using the 10x Genomics Chromium system. (B) Partitioning of cells to specific tissue types based on the combinatorial expression of known markers. (C,E) Bubble charts that depict the expression of markers used to identify DV domains of progenitors (C) and neuronal classes (E). Circle size indicates normalised gene expression levels. Genes selected for cell categorisation are coloured; grey circles correspond to markers not used for the selection of a specific population. (D) tSNE plot of the entire dataset based on transcriptional similarity using the same markers as B, coloured by assigned cell type. Neural progenitors (yellow) and neurons (orange) were selected for further analysis.