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. 2019 Mar 27;146(12):dev173807. doi: 10.1242/dev.173807

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Spatial and temporal patterns of gene expression in neural progenitors and neurons. (A) Identification of differentially expressed genes that encode cell adhesion molecules, TFs, and proteins that are involved in neurotransmission. Genes used in the initial partitioning of cell types are not shown. (B) Spatial and temporal expression of Cldn3 in neural progenitors and neurons in the dataset. Cldn3 is specifically expressed in MNs until e10.5. (C) Immunostaining at e10.5 for Cldn3 (green), Isl1 (blue), Mnx1 (red) and Olig2 (grey). (D) Cldn3 expression is specific to MNs at e10.5, and lost in MNs at e11.5 and e12.5. (E) Pou3f1 is expressed in dorsal d1-3 neurons at e11.5. dI1 neurons are labelled by Lhx2 expression and dI3 neurons by Isl1 expression. Scale bars: 100 µm.