Fig. 1.

Single cell RNA-seq identification of five major cell types at the lambdoidal junction. (A) Schematic showing process of microdissection of E11.5 lambdoidal junction (red, λ junction) and scRNA-seq experiment. The double-headed arrows indicate the anatomical axes relative to whole embryo (left) or the dissected prominences (right). (B) tSNE plot of 7893 cells from the C57LJ dataset showing the five major clusters. (C) Feature plots of representative marker genes used to assign clusters shown in B, with normalized gene expression per cell (colour key on the left) superimposed on the tSNE plot. The same colour key occurs in all subsequent feature plots. LNP, lateral nasal process; MNP, medial nasal process; MxP, maxillary prominence.