Fig. 2.

Heterogeneity in developmental timing and cell cycle within Dictyostelium mounds. (A) PCA of single cell transcriptomes reveals that developmental timing is highly heterogeneous in mounds. Shown here are the first two principal components. Each dot is a cell, coloured by the average expression value (z-score) of a set of genes that are upregulated (left panel) or downregulated during the mound stage (right panel). The gene set was taken from Rosengarten et al. (2015). PC1, and to a certain extent PC2, contains information about developmental advancement. (B) Same as A, but cells are coloured by the average expression value of a set of cell cycle-related genes. Differences in cell cycle-related gene expression are also captured by PC1 and PC2, which suggests that cell cycle advancement is not independent of developmental stage. (C) Vectors of developmental progression and the cell cycle state of cells in PC2/PC1 space.