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. 2019 Feb 21;11(11):10481–10491. doi: 10.1021/acsami.8b21398

Figure 4.

Figure 4

LNPLH transfection of a human cancer cell line panel with the bicistronic mRNA LNPLH/mRNA complex was prepared using the 20:100 w/w ratio of mRNA/LNP and used to transfect lung cancer cell lines (A549, H1650, HCC827, H1975, and HCC4006), a fibrosarcoma cell line (HT1080), prostate cancer cell lines (PC3 and DU145), colorectal cell lines (DLD1 and SW480), a Ewing Sarcoma cell line (A673), a breast cancer cell line (SKBR3), a pancreatic cancer cell line (PSN1), and embryonic kidney cells HEK293T. The percentage of cells with GFP fluorescence was analyzed using flow cytometry and the values converted to percentages of the maximum count using FlwoJo software as shown in the histograms. Lipofectamine 2000 was used as control for mRNA transfection. Data shown as mean values ± standard deviation of 3 or more samples.