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. 2019 May 20;47(W1):W171–W174. doi: 10.1093/nar/gkz365

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — The workflow of CHOPCHOP when targeting RNA for knock-down. The CHOPCHOP homepage (upper box) require four types of input: (i) target, (ii) species, (iii) CRISPR effector and (iv) the purpose of the experiment. Default options will be adequate for most users, but advanced options can be revealed using the ‘Options’ button. The results of the search (lower box) are displayed along with all isoforms of the target gene. The target color indicates the quality of each sgRNA or nickase pair (green [best] to red [worst]). Below the graphic representation an interactive table allows for exploring each guide in greater detail.