KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-CD3 | Dako | Cat#:A0452; RRID: AB_2335677 |
| Rabbit monoclonal clone SP239 anti-CD8 (for human IF) | Spring Biosciences | Cat#:M5390; RRID: AB_2756374 |
| Mouse monoclonal ant-CD163 | Leica | Cat#:NCL-L-CD163; RRID: AB_2756375 |
| Mouse monoclonal Clone AE1/AE3 anti-PanCK | Dako | Cat#:M3515; RRID: AB_2132885 |
| Rabbit polyclonal anti-DHODH | Proteintech | Cat#:14877-1-AP; RRID: AB_2091723 |
| Rabbit polyclonal anti-UMPS | Proteintech | Cat#:14830-1-AP; RRID: AB_2212392 |
| Rabbit polyclonal anti-VDAC1 | Abcam | Cat#:ab15895; RRID: AB_2214787 |
| Rabbit polyclonal anti-DCK | ThermoFisher Scientific | Cat#:PA5-21846; RRID: AB_11152040 |
| Rat M1/70 monoclonal anti-CD11b APC Cy7 | BD Pharmingen | Cat#:557657; RRID: AB_396772 |
| Rat 30-F11 monoclonal anti-CD45 Pacific Orange | ThermoFisher Scientific | Cat#:mcd4530; RRID: AB_2539700 |
| Rat BM8 monoclonal F4/80 PE-Cy5 | eBioscience | Cat#:15-4801-82; RRID: AB_468798 |
| Rabbit polyclonal anti-Ki67 | Abcam | Cat#:ab15580; RRID: AB_443209 |
| Rabbit monoclonal 5A1E anti-Cleaved Caspase 3 | Cell Signaling | Cat#:9664; RRID: AB_2070042 |
| Rabbit monoclonal 20E3 Histone H2A.X phosphoS139 | Cell Signaling | Cat#:9718; RRID: AB_2118009 |
| Rabbit monoclonal D4W2Z anti-CD8 (for mouse IHC) | Cell Signaling | Cat#:98941; RRID: AB_2756376 |
| Rabbit monoclonal E1E9V anti-Vinculin | Cell Signaling | Cat#:13901; RRID: AB_2728768 |
| Rat monoclonal 2.43 anti-CD8 (for in vivo depletion) | BioXcell | Cat#:BE0061; RRID: AB_1125541 |
| Anti-Rabbit IgG HRP | Cell Signaling | Cat# 7074; RRID: AB_2099233 |
| Biological Samples | ||
| Neoadjuvant Gemcitabine Treated PDA Patient FFPE Blocks | University of Michigan Department of Pathology | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| AR9 buffer | Perkin Elmer | Cat#:AR9001KT |
| 3H-Gemcitabine | Moravek Biochemicals | Cat#:MT1572 |
| Uniformly labeled 13C-glucose | Cambridge Isotopes | Cat#:CLM-1396 |
| Gemcitabine HCl | Cayman Chemical | Cat#:9003096 |
| RIPA buffer | Sigma Aldrich | Cat#:R0278 |
| cOmplete EDTA-Free | Sigma Aldrich | Cat#:11873580001 |
| Doxycycline Hyclate | Sigma Aldrich | Cat#:D9891 |
| 5-azacytidine | Cayman Chemical | Cat#:11164 |
| Decitabine | Cayman Chemical | Cat#:11166 |
| Fialuridine | Cayman Chemical | Cat#:15867 |
| Trifluridine | Cayman Chemical | Cat#:21366 |
| Capecitabine | Cayman Chemical | Cat#:10487 |
| 5-fluorouracil | Cayman Chemical | Cat#:14416 |
| 6-Aminonicotinamide | Sigma Aldrich | Cat#:A68203 |
| 2-deoxyglucose | Sigma Aldrich | Cat#:D3179 |
| FCCP | Sigma Aldrich | Cat#:C2920 |
| Oligomycin | Sigma Aldrich | Cat#:75351 |
| Rotenone | Sigma Aldrich | Cat#:R8875 |
| Antimycin A | Sigma Aldrich | Cat#:A8674 |
| Etomoxir | Sigma Aldrich | Cat#:E19G5 |
| AZD7507 | AstraZeneca | N/A |
| LPS | Enzo Life Sciences | Cat#:ALX-581-G11-LGG1 |
| IL6 | Peprotech | Cat#:216–16 |
| IL4 | Peprotech | Cat#:214–14 |
| MCSF | Peprotech | Cat#:315-G2 |
| Critical Commercial Assays | ||
| Vectra Stain ABC Kit | Vector Labs | Cat#:PK-61GG; RRID: AB_2336819 |
| DAB | Vector Labs | Cat#:SK-41GG; RRID: AB_2336382 |
| Opal 7-Color Manual IHC Kit | Perkin Elmer | Cat#:NEL811GG1KT |
| Lipofectamine RNAiMAX | ThermoFisher Scientific | Cat#:13778G75 |
| MycoAlert | Lonza | Cat#:LTG7–318 |
| Cell Titer Glo 2.0 | Promega | Cat#:G9241 |
| Experimental Models: Cell Lines | ||
| Mouse: KPC MT-3 | Gift from Dr. David Tuveson | N/A |
| Mouse: KPC MT-5 | Gift from Dr. David Tuveson | N/A |
| Mouse: KPC28258 | Gift from Dr. Sunil Hingorani | N/A |
| Mouse: KPC 7940 | Gift from Dr. Gregory Beatty | N/A |
| Human: MiaPaCa2 | ATCC | Cat#:CRL-142G; RRID: CVCL_0428 |
| Human: Panc1 | ATCC | Cat#:CRL-1469; RRID: CVCL_0480 |
| Human: BXPC3 | ATCC | Cat#:CRL-1687; RRID: CVCL_0186 |
| Mouse: L929 | ATCC | Cat#:CCL-1; RRID: CVCL_0462 |
| Mouse: RAW264.7 | ATCC | Cat#:SC-6003 |
| Human: PA-TU-8902 | DSMZ | Cat#:ACC 179; RRID: CVCL_1845 |
| Human: UM18 | Generated as in Li et al., 2007 | N/A |
| Human: UM90 | Generated as in Li et al., 2007 | N/A |
| Human: UM143 | Generated as in Li et al., 2007 | N/A |
| Mouse: iKras*3 | Zhang et al., 2017a | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | Jackson Laboratory | Cat#: 000664; RRID: IMSR_JAX:000664 |
| Mouse: B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J | Jackson Laboratory | Cat#: 006000; RRID: IMSR_JAX:006000 |
| Mouse: Kras+/LSL-G12D;Trp53+/LSL-R172H;Pdx1-Cre | Hingorani et al. (2005) | N/A |
| Oligonucleotides | ||
| ON-TARGETplus siNT | Dharmacon | Cat#:D-001810–10-05 |
| ON-TARGETplus siDhodh | Dharmacon | Cat#:L-063269–01-0005 |
| ON-TARGETplus siUmps | Dharmacon | Cat#:L-042834–01-0005 |
| Software and Algorithms | ||
| Metabolanalyst | http://www.metaboanalyst.ca/ | N/A |
| Halo | Indica Labs | http://www.indicalab.com/halo/ |
| Bioconductor | https://www.bioconductor.org/ | RRID:SCR_006442 |
| Prism 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/; RRID: SCR_002798 |
| MassHunter Workstation Quantitative Analysis Software | Agilent | https://www.agilent.com/en/products/software-informatics/masshunter-suite/masshunter-quantitative-analysis |
| MassHunter Workstation Software Profinder | Agilent | https://www.agilent.com/en/products/software-informatics/masshunter-suite/masshunter-for-life-science-research/profinder-software |