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. 2019 Jun 4;16(8):1001–1009. doi: 10.1080/15476286.2019.1620061

Figure 4.

Figure 4.

Depletion of SNRPA/SNRNP70/SNRPD2 led to cytoplasmic accumulation of MEG3 reporter transcripts and enrichment of endogenous lncRNAs in cytoplasm.

A. Western blot showing knockdown efficiency after treatment of HeLa cells with specific siRNA. B. RNA-FISH showing depletion of SNRPA/SNRNP70/SNRPD2 resulted in shift of MEG3 reporter transcripts from nucleus to cytoplasm. C. The percentage of cells with apparent FISH signal in cytoplasm increased significantly after depletion of SNRPA/SNRNP70/SNRPD2. (n = 3) ***: p < 0.001. D. RT-PCR results showing elevated distribution of MEG3 reporter transcripts in cytoplasm after depletion of SNRPA/SNRNP70/SNRPD2. (n = 3) *: p < 0.05; **: p < 0.01; ***: p < 0.001. E. Western blot showing knockdown efficiency after treatment of HFF1 with siRNAs against SNRPA/SNRNP70/SNRPD2 simultaneously. Si-3KD: triple knockdown of SNRPA, SNRNP70 and SNRPD2. F. RT-PCR showing distribution of endogenous lncRNAs in cytoplasm and nucleus after siRNA treatment of HFF1 cells. C: cytoplasm; N: nucleus; W: whole cell. G. Quantification of lncRNAs localized in the cytoplasm. (n = 3) *: p < 0.05; **: p < 0.01; ***: p < 0.001.