Table 4. Cryo-EM data collection, refinement, and validation.
| CTF3c (EMDB-20200) (PDB 6OUA) |
|
|---|---|
| Data collection and processing | |
| Magnification | 105,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 51 |
| Defocus range (μm) | −1.5 to −3.5 |
| Pixel size (Å) | .85 |
| Symmetry imposed | None |
| Initial particle images (no.) | 2,062,207 |
| Final particle images (no.) | 71,632 |
| Map resolution (Å) FSC threshold |
4.18 (0.143) |
| Refinement | |
| Initial model used (PDB code) | 6NUW |
| Model resolution (Å) FSC threshold |
4.24 (0.5) |
| Model resolution range (Å) | 40–4.18 |
| Map sharpening B factor (Å2) | −151.424 |
| Model composition Non-hydrogen atoms Protein residues Ligands |
5208 637 0 |
| B factors (Å2) | Min: 36.02 Max: 141.08 Mean: 72.17 |
| R.m.s. deviations Bond lengths (Å) Bond angles (°) |
0.008 1.351 |
| Validation MolProbity score Clashscore Poor rotamers (%) |
2.37 7.09 2.35 |
| Ramachandran plot Favored (%) Allowed (%) Disallowed (%) |
82.25 99.68 0.32 |