Table 2. HPLC purification conditions for the peptides used in this study.

Peptide	HPLC column	Flow rate	Gradient
F	Phenomenex C4 Luna (100 Å, 10 μm, 10 × 250 mm)	5 mL/min	20 to 80% Buffer B
F12	Phenomenex C12 Jupiter (90 Å, 10 μm, 21.2 × 250 mm)	5 mL/min	25 to 45% Buffer B
F30	Phenomenex C12 Jupiter (90 Å, 10 μm, 21.2 × 250 mm)	10 mL/min	20 to 45% Buffer B
cF30 (pre-cyclization)	Phenomenex C12 Jupiter (90 Å, 10 μm, 21.2 × 250 mm)	10 mL/min	20 to 45% Buffer B
cF30	Phenomenex C4 Jupiter (100 Å, 10 μm, 10 × 250 mm)	5 mL/min	10 to 35% Buffer B
FF30	Phenomenex C12 Jupiter (90 Å, 10 μm, 21.2 × 250 mm)	10 mL/min	29 to 37% Buffer B
cFF30 (pre-cyclization	Phenomenex C12 Jupiter (90 Å, 10 μm, 21.2 × 250 mm)	10 mL/min	20 to 45% Buffer B
cFF30	Phenomenex C4 Jupiter (100 Å, 10 μm, 10 × 250 mm)	5 mL/min	27 to 38% Buffer B