Fig 2.

R848 induces lymphocyte migration in zebrafish gills. A and C, Maximum z-stack projections of Tg(lyz:GFP; neutrophils; Fig 2, A) and Tg(lck:GFP; lymphocytes; Fig 2, C) gills after treatment with water or R848 for 3 (Fig 2, A) and 8 (Fig 2, C) hours (n = 7). Scale bars = 100 μm. B and D, Average number of GFP⁺ cells in the first 20 lamellae of each filament. Each dot indicates average counts per individual fish (n ≥ 6). Values are presented as means ± SEMs. Two-way ANOVA followed by the Sidak multiple comparison test was used.