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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Alcohol Clin Exp Res. 2019 May 14;43(7):1439–1451. doi: 10.1111/acer.14065

Figure 5. Timecourse of UPR induction in the liver after Gao-Binge feeding and UPR induction in AH patients.

Figure 5

C57BL/6J mice were euthanized at 0h-9h after ethanol binge per Gao-Binge ethanol feeding and UPR was determined A-B) Expression of phosphorylated eIF2α, CHOP and GRP78 were determined by western blot. Densitomtery was carried out with β-Actin as a loading control. C) Expression of UPR-associated Chop, Grp78, Fgf21 and spliced XBP-1 mRNA was determined by qRT-PCR. Gene expression was normalized to 18S RNA and expressed as fold change over pair-fed controls. D) UPR activation in livers of AH patients was determined by western blot. Expression of phosphorylated eIF2α, CHOP, PDI and GRP78 was normalized to β-actin and represented as fold over healthy controls. E) Expression of Chop and Grp78 mRNA was determined by qRT-PCR and normalized to 18S RNA. Values are means ± SEM. Values with different superscripts are significantly different, p<0.05. # p<0.05 vs. HC or PF.