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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Alcohol Clin Exp Res. 2019 May 14;43(7):1439–1451. doi: 10.1111/acer.14065

Figure 6. MIF delays the binge-associated UPR in mice.

Figure 6

UPR was determined in livers of A-D) C57BL/6J or Mif-/- as well as E-H) C57BL/6J mice treated with MIF098 or VEH at 6h and 9h after Gao-Binge. A,C,E,G) Phosphorylation of eIF2α, expression of CHOP and GRP78 were determined by western blot and normalized to β-Actin as a loading control at the indicated times. B,D,F,H) Expression of spliced XBP-1, CHOP, Grp78, Dr5, and FGF21 mRNA was determined by qRT-PCR and normalized to 18S RNA and represented as fold change over pair-fed controls at the indicated times. Values are means ± SEM. Values with different superscripts are significantly different, p<0.05, # - p<0.05 vs pair-fed.